Methodological Comparison of Field And Laboratory Sample Processing for Chlorophyll-A Measurement in Two Tropical Resevoir

Desty Pratiwi; Sunardi Sunardi; Miranti Ariyani; Keukeu Kaniawati Rosada

doi:10.51264/inajl.v7i1.133

Authors

Desty Pratiwi Research Center for Limnology and Water Resources, National Research and Innovation Agency, Cibinong, Indonesia
Sunardi Sunardi Department of Biology, Faculty of Science and Mathematics, Universitas Padjadjaran, Bandung, Indonesia
Miranti Ariyani Research Center for Environment and Clean Technology, National Research and Innovation Agency, Bandung, Indonesia
Keukeu Kaniawati Rosada Department of Biology, Faculty of Science and Mathematics, Universitas Padjadjaran, Bandung, Indonesia

DOI:

https://doi.org/10.51264/inajl.v7i1.133

Keywords:

Cirata reservoir, Jatigede Reservoir, Plankton net, Technique sampling, Trophic status

Abstract

Chlorophyll-a is widely used as an indicator of phytoplankton biomass and trophic condition. However, limited information is available on how sample volume and processing location affect chlorophyll-a measurements in tropical reservoirs. This study compared three sampling procedures in Cirata and Jatigede Reservoirs, West Java, Indonesia. The method compared a 1-L sample concentrated in the laboratory (1NF), a 1-L sample concentrated in the field using a 25-µm plankton net (1PN), and a 10-L sample concentrated in the field using the same net (10PN). Chlorophyll-a was extracted using 90% acetone and measured by UV–Vis spectrophotometry. In Cirata Reservoir, chlorophyll-a concentrations were 5.470, 3.333, and 1.228 µg/L for 1NF, 1PN, and 10PN, respectively. In Jatigede Reservoir, the corresponding values were 19.380, 24.967, and 4.370 µg/L. The 10PN treatment produced the lowest chlorophyll-a values in both reservoirs, while the response of the 1PN treatment differed between reservoirs. These findings indicate that sample volume and processing location may influence chlorophyll-a estimates. Further validation using a standard direct-filtration method and adequate replication is needed before one procedure can be recommended as the most accurate.

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